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Improved chip design for integrated solid-phase microextraction in on-line proteomic sample preparation

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Author

Summary, in English

A recently introduced silicon microextraction chip (SMEC), used for on-line proteomic sample preparation, has proved to facilitate the process of protein identification by sample clean up and enrichment of peptides. It is demonstrated that a novel grid-SMEC design improves the operating characteristics for solid-phase microextraction, by reducing dispersion effects and thereby improving the sample preparation conditions. The structures investigated in this paper are treated both numerically and experimentally. The numerical approach is based on finite element analysis of the micro-fluidic flow in the microchip. The analysis is accomplished by use of the computational fluid dynamics-module FLOTRAN in the ANSYS(R) software package. The modeling and analysis of the previously reported weir-SMEC design indicates some severe drawbacks, that can be reduced by changing the microextraction chip geometry to the grid-SMEC design. The overall analytical performance was thereby improved and also verified by experimental work. Matrix-assisted laser desorption/ionization mass spectra of model peptides extracted from both the weir-SMEC and the new grid-SMEC support the numerical analysis results. Further use of numerical modeling and analysis of the SMEC structures is also discussed and suggested in this work.

Publishing year

2002

Language

English

Pages

422-429

Publication/Series

Proteomics

Volume

2

Issue

4

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Electrical Engineering, Electronic Engineering, Information Engineering

Keywords

  • sample preparation
  • solid phase
  • fluid dynamics

Status

Published

ISBN/ISSN/Other

  • ISSN: 1615-9861