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Polyamine Depletion with Two Different Polyamine Analogues Causes DNA Damage in Human Breast Cancer Cell Lines.

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Author

Summary, in English

It is well known that the positively charged polyamines have a DNA-stabilizing function and that polyamine depletion alters chromatin function. We have previously shown that polyamine depletion causes an S phase prolongation, and others have shown that there is an accumulation of Okazaki-like fragments in polyamine-depleted cells. In the present study we have used the comet assay to investigate polyamine depletion-induced DNA strand breaks. Three breast cancer cell lines and one normal-like breast cell line were treated with the polyamine analogue N(1),N(11)-diethylnorspermine or with the polyamine biosynthesis inhibitor 4-amidinoindan-1-one 2'-amidinohydrazone (CGP 48664). The comet assay showed that polyamine depletion resulted in DNA strand breaks. We also show that these DNA strand breaks occurred in cells where there was no expression of gamma-H2AX, which is a marker of DNA double-strand breaks. Thus, our conclusion is that polyamine depletion causes DNA single-strand breaks, which may be the cause for the observed delay in S phase progression.

Publishing year

2008

Language

English

Pages

511-516

Publication/Series

DNA and Cell Biology

Volume

27

Issue

9

Document type

Journal article

Publisher

Mary Ann Liebert, Inc.

Topic

  • Zoology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1044-5498