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Pasteurella multocida thymidine kinase 1 efficiently activates pyrimidine nucleoside analogs.

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Author

  • Anders Ranegaard Clausen
  • S A L Al Meani
  • Jure Piskur

Summary, in English

In the Pasteurella multocida genome only one putative deoxyribonucleoside kinase encoding gene, for thymidine kinase 1 (PmTK1), was identified. The PmTK1 gene was sub-cloned into Escherichia coli KY895 and it sensitized the host towards 2',2'-difluoro-deoxycytidine (gemcitabine, dFdC), 3'-azido-thymidine (AZT) and 5-fluoro-deoxyuridine (5F-dU). PmTK1 was over-expressed and purified with two different tags. Apparently, deoxyuridine (dU), and not thymidine (dT), is the preferred substrate. We suggest that PmTK1s could be employed as a species-specific activator of uracil-based nucleoside antibiotics.

Publishing year

2010

Language

English

Pages

359-362

Publication/Series

Nucleosides, Nucleotides & Nucleic Acids

Volume

29

Issue

4-6

Document type

Journal article

Publisher

Taylor & Francis

Topic

  • Biological Sciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 1525-7770