On Lactobacillus plantarum 299v, bacterial translocation and intestinal permeability.
Author
Summary, in English
In study I L. plantarum 299v normalized E. coli-induced permeability in distal ileum of rats. This effect was achieved with a more continuous supply of L. plantarum 299v, rather than intermittent or acute pretreatment.
Study II showed that pretreatment with L. plantarum 299v prevented BT in rats rendered septic with LPS. The protective effect seemed to be dependent on the ability of L. plantarum 299v to adhere to intestinal mucosa, indicating competitive inhibition as a possible mechanism behind prevention of translocation. Moreover, treatment with prebiotics, without a probioticum, did not prevent translocation.
In study III LPS-induced intestinal barrier dysfunction on rats was found to be regulated by P-selectin-dependent leukocyte recruitment. With anti-P-selectin antibody intestinal permeability was attenuated, as well as leukocyte rolling and adhesion. This indicates anti-P-selectin treatment as a mean of ameliorating barrier dysfunction in sepsis.
Finally, in study IV, it was found that high doses of L. plantarum 299v given to patients undergoing colon surgery were not able to reduce translocation of selected enteric bacteria but seemed to have a stimulatory effect on bacterial load in the colon. Further, L. plantarum 299v could be given to patients with malignancy without risk of tumour proliferation.
Taken together, the findings herein indicates a potential of L. plantarum 299v pretreatment in reducing BT and intestinal permeability and warrants further studies in the clinical setting.
Department/s
Publishing year
2007
Language
English
Publication/Series
Lund University Faculty of Medicine Doctoral Dissertation Series
Volume
2007:17
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Document type
Dissertation
Publisher
Department of Surgery, Malmö University Hospital, Lund University
Topic
- Surgery
Keywords
- bacterial translocation
- permeability
- intestinal microflora
- Kirurgi
- orthopaedics
- P-selectin
- ortopedi
- traumatologi
- leukocyte recruitment
- Lactobacillus plantarum 299v
- traumatology
- Surgery
- colon resection
Status
Published
Research group
- Surgery
Supervisor
ISBN/ISSN/Other
- ISSN: 1652-8220
- ISBN: 91-85559-85-7
Defence date
8 February 2007
Defence time
09:00
Defence place
Lecture hall, Dept of Surgery, Malmö University Hospital, entrance 42
Opponent
- Johan Söderholm (Associate professor)