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In vitro molecular evolution of antibody genes mimicking receptor revision

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Author

Summary, in English

Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved.

Publishing year

2002

Language

English

Pages

349-356

Publication/Series

Molecular Immunology

Volume

39

Issue

5-6

Document type

Journal article

Publisher

Pergamon Press Ltd.

Topic

  • Immunology in the medical area

Keywords

  • phage display
  • affinity maturation
  • antibody
  • receptor revision
  • somatic hypermutation

Status

Published

ISBN/ISSN/Other

  • ISSN: 1872-9142