Design of high-density antibody microarrays for disease proteomics: Key technological issues.
Author
Summary, in English
Antibody-based microarray is a novel proteomic technology setting a new standard for molecular profiling of non-fractionated complex proteomes. The first generation of antibody microarrays has already demonstrated its potential for generating detailed protein expression profiles, or protein atlases, of human body fluids in health and disease, paving the way for new discoveries within the field of disease proteomics. The process of designing highly miniaturized, high-density and high-performing antibody microarray set-ups have, however, proven to be challenging. In this mini-review we discuss key technological issues that must be addressed in a cross-disciplinary manner before true global proteome analysis can be performed using antibody microarrays.
Department/s
Publishing year
2009
Language
English
Pages
928-935
Publication/Series
Journal of Proteomics
Volume
72
Issue
6
Links
Document type
Journal article
Publisher
Elsevier
Topic
- Immunology in the medical area
Status
Published
ISBN/ISSN/Other
- ISSN: 1874-3919