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A new expression vector for production of enzymes in the yeast Saccharomyces (lachancea) kluyveri.

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Author

Summary, in English

We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.

Publishing year

2011

Language

English

Pages

1227-1232

Publication/Series

Nucleosides, Nucleotides & Nucleic Acids

Volume

30

Issue

12

Document type

Journal article

Publisher

Taylor & Francis

Topic

  • Biological Sciences

Keywords

  • Expression vector
  • Saccharomyces (Lachancea) kluyveri
  • URC pathway
  • catabolism of pyrimidines

Status

Published

ISBN/ISSN/Other

  • ISSN: 1525-7770