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A new approach to examine conformational changes occurring upon binding of ligand by biomolecules

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Author

  • Christer Wingren
  • Ulla-Britt Hansson

Summary, in English

Liquid-liquid partition chromatography in an aqueous poly(ethylene glycol)/dextran two-phase system (LLPC) is shown to be a quick and sensitive method for detecting conformational changes occurring upon binding of ligands by biospecific molecules. Two groups of well-characterized proteins, enzymes and monoclonal antibodies, were employed. As an example, LLPC demonstrated that isoforms of lactate dehydrogenase as well as of hexokinase existed in a ligand-dependent equilibrium between two forms and that conformational changes occurred when monoclonal antibodies bound haptens. We also demonstrate that the method could be used to detect and separate subfractions in preparations of unliganded proteins that appeared to be homogeneous when analysed by other techniques.

Publishing year

1995

Language

English

Pages

209-215

Publication/Series

Biochimica et Biophysica Acta. General Subjects

Volume

1244

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • Conformational change
  • Phase partition
  • LLPC
  • Ligand binding
  • Antibody
  • Enzyme

Status

Published

ISBN/ISSN/Other

  • ISSN: 0304-4165