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Genetic Analysis of Cytosolic PGI in Festuca ovina

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Author

Summary, in English

<i>Festuca ovina</i>, sheep's fescue is a widely distributed perennial outbreeder that belongs to the grass family Poaceae and is very common in the northern temperate zone. The genus <i>Festuca s.l.</i> is partitioned into fine-leaved fescues to which <i>F. ovina</i> belongs, and broad-leaved fescues to which the genus <i>Lolium</i> is a close relative. Wide crosses within the <i>Festuca-Lolium</i> complex often yield fertile hybrids. My thesis describes a genetic analysis of the variation for cytosolic phosphoglucose isomerase (PGI) in <i>Festuca ovina</i>. The investigation was initiated since an isozyme study demonstrated that some plants had multibanded electrophoretic patterns that proved genetically uninterpretable. Chromosome analysis showed that the plants were not polyploid, but diploid with the standard number of 14 chromosomes. Classical genetic analysis based on artificial crosses revealed that a second PGI locus segregated in south-Swedish populations of <i>F. ovina</i>. The second locus (<i>PgiC2</i>) and the standard locus (<i>PgiC1</i>) assorted independently, <i>i.e.</i> were not linked. Sequence data revealed that the two loci were highly diverged. Moreover, <i>PgiC2</i> proved to be more complex than <i>PgiC1</i>, since all three <i>PgiC2</i> variants analysed were composed of two genes. One of these genes may be a pseudogene. The results demonstrate that <i>PgiC2</i> is not a simple, duplicated version of <i>PgiC1</i>. The sequences present today at these two loci must have started to diverge a long time ago, perhaps as far back as the time of the differentiation of the genus. I suggest that <i>PgiC2</i> entered <i>F. ovina</i> recently via an introgression event from another species in the <i>Festuca-Lolium</i> complex, where it normally functions as the "standard" locus for cytosolic PGI. This model is supported by the fact that alleles from <i>PgiC1</i> and <i>PgiC2</i> produce enzyme subunits that easily function together despite their wide sequence divergence. Thus, my thesis brings into focus a possible but not much discussed way for eukaryot organisms to add new genes to their genomes.

Department/s

  • MEMEG
  • Evolutionary Genetics

Publishing year

2003

Language

English

Document type

Dissertation

Publisher

Lena Ghatnekar, Dept. of Cell and Organism Biology, Lund University, Sweden,

Topic

  • Biological Sciences

Keywords

  • Växtgenetik
  • Plant genetics
  • pseudogenes
  • grasses
  • cytosolic PGI
  • introgression
  • duplication
  • Festuca ovina

Status

Published

Research group

  • Evolutionary Genetics

Supervisor

  • [unknown] [unknown]

ISBN/ISSN/Other

  • ISBN: 91-85067-07-5

Defence date

29 November 2003

Defence time

10:00

Defence place

Genetics building, Sölvegatan 29, Lund

Opponent

  • Deborah Charlesworth (Professor)