Immobilisation of DNA to polymerised SU-8 photoresist

Summary, in English

SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm2 as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 °C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA microarrays of high quality were obtained on SU-8 by contact printing of probe solution directly on SU-8 demonstrating a simple method for the implementation of microarrays in microsystems.

• Author: Rodolphe Marie; Silvan Schmid; Alicia Johansson; Louise Ejsing; Maria Nordström; Daniel Häfliger; Claus BV Christensen; Anja Boisen; Martin Dufva
• Publishing year: 2006
• Language: English
• Pages: 1327-1332
• Publication/Series: Biosensors & Bioelectronics
• Volume: 21
• Issue: 7
• Links: http://dx.doi.org/10.1016/j.bios.2005.03.004
• Document type: Journal article
• Publisher: Elsevier
• Topic: Medical Engineering
• Status: Published
• ISBN/ISSN/Other: ISSN: 1873-4235