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Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex

Author

  • Veronika Fekete
  • Maria Cierna
  • Silvia Poláková
  • Jure Piskur
  • Pavol Sulo

Summary, in English

Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species.

Publishing year

2007

Language

English

Pages

1237-1247

Publication/Series

FEMS Yeast Research

Volume

7

Issue

8

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Biological Sciences

Keywords

  • ethidium
  • Saccharomyces/Kluyveromyces complex
  • petite mutation
  • mtDNA
  • bromide
  • mitochondria

Status

Published

ISBN/ISSN/Other

  • ISSN: 1567-1364