Galectin binding proteins in serum and bronchoalveolar lavage -in healthy and pathological conditions

Title	Galectin binding proteins in serum and bronchoalveolar lavage -in healthy and pathological conditions
Author/s	Cecilia Cederfur
Department/s	Division of Microbiology, Immunology and Glycobiology - MIG
Full-text	Available as PDF
Defence date	2008-05-31
Defence time	09:00
Defence place	Segerfalksalen, Wallenberg neurocentrum, BMC, Sölvegatan 17
Opponent	professor Peter Påhlsson
Publication/Series	Lund University, Faculty of Medicine Doctoral Dissertation Series
Publishing year	2008
Volume	2008:68
Pages	73
Document type	Dissertation
Language	English
Publisher	Dept of Laboratory Medicine, Lund University
Abstract English	Galectins are carbohydrate binding proteins, implicated in conditions of both inflammation and cancer. Connections between chronic inflammation and cancer are proposed by the increased remodelling and proliferation that occurs, leading to enhanced survival and proliferation of malignant cells. Since galectins have been implicated in mechanisms of both chronic inflammation and cancer, we have investigated natural binding partners of galectins in healthy individuals and then continued with studying states of cancer and chronic inflammation. We identified galectin binding glycoproteins in sera from healthy individuals and found that galectins widely expressed in the body bind serum glycoproteins well, whereas galectins with a more tissue-specific distribution scarcely binds any serum glycoproteins. We then chose the widely expressed but intermediately binding galectin-1 to detect if levels of galectin-1-binding proteins are increased in sera of breast cancer patients. We found that galectin-1 binds approximately double the amount in breast cancer patients compared to healthy individuals. The increase was mainly caused by haptoglobin, probably due to both increased expression and changes of glycosylation. To further investigate the inflammatory connection we identified galectin binding proteins from bronchoalveolar lavage of asthma patients and healthy individuals, additionally we compared the binding of galectin-3 and galectin-8 that are expressed in different sites of the lung. We found when functionally grouping the bound proteins that galectin-3 and -8 binding proteins had different profiles and that bound proteins of healthy and asthma patients differed.
Subject	Medicine and Health Sciences
Keywords	glycoprotein, cancer, asthma, bronchoalveolar lavage, galectin binding protein, galectin ligand, galectin, serum
ISBN/ISSN/Other	ISSN: 1652-8220 ISBN: 978-91-86059-21-7
Supervisor	Hakon Leffler
References	References
Part of	Different affinity of galectins for human serum glycoproteins: Galectin-3 binds many protease inhibitors and acute phase proteins Description: published manuscript Restriction: Free access

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