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| Title | Electron-Transfer Studies with a New Flavin Adenine Dinucleotide Dependent Glucose Dehydrogenase and Osmium Polymers of Different Redox Potentials |
| Author/s | MNadeem Zafar, Xiaoju Wang, Christoph Sygmund, Roland Ludwig, Donal Leech, Lo Gorton |
| Department/s |
Analytical Chemistry (S/LTH)
|
| Full-text | Full text is not available in this archive |
| Alternative location (URL) | http://dx.doi.org/10.1021/ac20... Restricted Access (Alternative Location) |
| Publication/Series | Analytical Chemistry |
| Publishing year | 2012 |
| Volume | 84 |
| Issue | 1 |
| Pages | 334 - 341 |
| Document type | Journal article |
| Status | published |
| Quality controlled | yes |
| Language | English |
| Publisher | American Chemical Society |
| Abstract English | A new extracellular flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase from Glomerella cingulata (GcGDH) was electrochemically studied as a recognition element in glucose biosensors. The redox enzyme was recombinantly produced in Pichia pastoris and homogeneously purified, and its glucose-oxidizing properties on spectrographic graphite electrodes were investigated. Six different Os polymers, the redox potentials of which ranged in a broad potential window between +15 and +489 mV versus the normal hydrogen electrode (NHE), were used to immobilize and "wire" GcGDH to the spectrographic graphite electrode's surface. The GcGDH/Os polymer modified electrodes were evaluated by chronoamperometry using flow injection analysis. The current response was investigated using a stepwisely increased applied potential. It was observed that the ratio of GcGDH/Os polymer and the overall loading of the enzyme electrode significantly affect the performance of the enzyme electrode for glucose oxidation. The best-suited Os polymer [Os(4,4'-dimethyl-2,2'-bipyridine)(2)(PVI)CI](+) had a potential of +309 mV versus NHE, and the optimum GcGDH/Os polymer ratio was 1:2 yielding a maximum current density of 493,mu A.cm(-2) at a 30 mM glucose concentration. |
| Subject |
Chemistry |
| ISBN/ISSN/Other |
ISSN: 0003-2700 |
