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| Title | Post-Ischemic Housing Conditions Influence On Gene Transcription And Translation After Permanent Focal Brain Ischemia In Rats |
| Author/s | Li-Ru Zhao |
| Department/s |
Laboratory for Experimental Brain Research
|
| Full-text | Available as PDF |
| Defence date | 2004-09-15 |
| Defence time | 10:15 |
| Defence place | Segerfalksalen, Wallenberg Neurocentrum |
| Opponent | Peter Eriksson |
| Publishing year | 2004 |
| Pages | 71 |
| Document type | Dissertation |
| Language | English |
| Publisher | Li-Ru Zhao, Dept of clinical neuroscience, Lund, Sweden, |
| Abstract English | Enriched environment (EE) housing significantly ameliorates neurological deficits induced by cortical brain ischemia without changing infarction size, suggesting that EE-related functional benefits are associated with neuronal plasticity events in the remaining tissue. Brain-derived neurotrophic factor (BDNF), nerve growth factor-induced gene A (NGFI-A) and corticosteroid receptors (mineralocorticoid receptor, MR; glucocorticoid receptor, GR) have been demonstrated to be involved in brain plasticity. The purpose of this thesis was to determine if post-ischemic housing conditions had a significant effect on transcription and/or translation of BDNF, NGFI-A and corticosteroid receptors. We found that BDNF gene was down regulated in EE-housed rats when compared to the rats housed in standard cages at 2~12 days after cortical brain ischemia in peri-infarct cortex, contralateral cortex and bilateral hippocampus. The protein level of BDNF in the ipsilateral frontal cortex was lower in the EE-housed rats than the standard environment (SE)-housed rats at 12d postischemia. The mRNA expression of NGFI-A showed a similar pattern of BDNF except for an increase at 30 d after induction of brain ischemia in EE-housed rats. Ischemia-induced reduction of GR was prevented in the rats housed in EE condition. There was no difference between EE- and SE-housed animals in MR gene expression. Gene expressions, however, are very complex in housing conditions and postischemia. Whether EE-related gene transcription and/or translation reported in this thesis are linked with EE-induced functional benefits to brain ischemia, further studies need be conducted. |
| Subject |
Medicine and Health Sciences |
| Keywords | brain-derived neurotrophic factor, rats, mRNA, nerve growth factor-induced gene A, corticosteroid receptors, in situ hybridization, Neurologi, neurophysiology, Neurology, neuropsychology, brain ischemia, enriched environment, neuropsykologi, neurofysiologi |
| ISBN/ISSN/Other |
ISBN: 91-628-6171-9 |
| Additional info | Article:I. Zhao LR, Mattsson B, Johansson BB. Environmental influence on brain-derived neurotrophic factor messenger RNA expression after middle cerebral artery occlusion in spontaneously hypertensive rats. Neuroscience. 2000;97:177-184. Article:II Zhao LR, Risedal A, Wojcik A, Hejzlar J, Johansson BB, Kokaia Z.Enriched environment influences brain-derived neurotrophic factor levels in rat forebrain after focal stroke. Neurosci Lett. 2001; 305: 169-172. Article:III. Dahlqvist P, Zhao L, Johansson IM, Mattsson B, Johansson BB, Seckl JR, Olsson T. Environmental enrichment alters nerve growth factor-induced gene A and glucocorticoid receptor messenger RNA expression after middle cerebral artery occlusion in rats. Neuroscience.1999; 93: 527-535. |
