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Variation among Colletotrichum gloeosporioides isolates from infected coffee berries at different locations in Vietnam

Author

Summary, in English

Isolates of Colletotrichum, gloeosporioides associated with anthracnose disease oil coffee berries in Vietnam were characterized by morphological and molecular methods. Random amplified polymorphic DNA (RAPD) and microsatellite-primered PCR (MP-PCR) analyses were employed to investigate the genetic variation among 38 and 51 isolates of C. gloeosporioides, respectively. According to both methods, the isolates mainly grouped in accordance with geographical origins. Higher genetic variation (H = 0.312 and 0.335) in the northern Population of C. gloeosporioides than ill the Southern population (H = 0.261 and 0.186), according to the RAPD and MP-PCR markets, respectively, was indicative of a difference between the northern and southern Populations. Moderate gene differentiation (Gst = 0.1) between Populations from the north and the south was found. However, there was no differentiation between locations within the northern or Southern populations, indicating significant gene flow. A four-gamete test indicated a high level of recombination, particularly Ill the South. The geographic differences may be explained by different histories of coffee cultivation in different parts of Vietnam. The symptoms caused by the Vietnamese isolates on both hypocotyls and green berries were less severe than symptoms caused by the reference CBD (coffee berry disease; Colletotrichum kahalvae) isolates originating from Africa.

Department/s

  • MEMEG
  • Evolutionary Genetics

Publishing year

2009

Language

English

Pages

898-909

Publication/Series

Plant Pathology

Volume

58

Issue

5

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Biological Sciences

Keywords

  • genetic variation
  • anthracnose disease
  • Coffea spp.
  • MP-PCR
  • pathogenicity
  • RAPD

Status

Published

Research group

  • Evolutionary Genetics

ISBN/ISSN/Other

  • ISSN: 1365-3059