Javascript is not activated in your browser. This website needs javascript activated to work properly.
You are here

Thylakoid enriched powder product Appenthyl is resistant against gastrointestinal digestion and keeps on inhibiting lipolysis

  • Manjuma Asheka
Publishing year: 2014
Language: English
Document type: Student publication for Master's degree (two years)


In earlier studies it was revealed that thylakoid membranes inhibit activity of pancreatic lipase/colipase. More studies have shown that these membranes induce satiety hormone CCK and reduce hunger hormone of rats and human. Appethyl® used in this project is processed from spinach leaves in such a way that it reserves most amounts of thylakoid membranes. The results, in this project, shows that Appethyl® can inhibit the activity of lipase/colipase after digestive treatment. Separate reactions indicate highest inhibition capacity of Appethyl® after pepsin and gastric juice digestion. The positive thing is that, Appethyl® shows inhibition capacity on lipase/colipase even after treatment with pepsin, trypsin, pepsin/ trypsin, gastric juice (GJ), pancreatic juice(PJ), gastric/pancreatic juice. Treatment with GJ followed by long time in PJ also does not affect the thylakoid membranes present in Appethyl®. This conclusion was based on SDS-PAGE electrophoresis gel picture with Appethyl® incubated 45 minutes in GJ and 0-6 hours in PJ. This is also supported by the result of inhibition capacity by Appethyl® as 36%- 31% after GJ /PJ treatment. SDS-PAGE gel treated and untreated Appethyl® showed that there were protein bands at the MW position for LCH II and Rubisco large chain and Rubisco small chain.


  • Chemistry
  • biokemi
  • biochemistry


  • Per-Åke Albertsson
  • Sinan Cem Emek