Tools for understanding the glycosaminoglycan biosynthesis
Author
Summary, in English
This thesis is focused on the design and synthesis of modified xylosides and how we can use these as tools to study the formation of GAGs. We decided to alter the carbohydrate itself to investigate if it is possible to design effective substrates or inhibitors for β4GalT7. We also decided to modify the aglycon, the part attached to the carbohydrate, to answer questions related to how cells process GAGs. This work has resulted in several new substances that, that enter cells, and work well as substrates and inhibitors of β4GalT7 to provide answers to how GAGs are structured, how they move within the cell, and how they interact with other biomolecules such as viral proteins. The results of this work pave the way for the use of synthetic xylosides to answer several questions about GAG-related processes and open up the possibility for creating tools for influencing and studying cells' expression of GAGs.
Department/s
Publishing year
2021
Language
English
Full text
- Available as PDF - 27 MB
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Document type
Dissertation
Publisher
Lund University, Faculty of Science, Department of Chemistry, Centre for Analysis and Synthesis
Topic
- Organic Chemistry
Keywords
- Xylosides
- Glycosaminoglycans
- Proteoglycans
- β4GalT7
- Carbohydrate synthesis
- GAG priming
- GAG inhibition
Status
Published
Supervisor
ISBN/ISSN/Other
- ISBN: 978-91-7422-847-2
- ISBN: 978-91-7422-846-5
Defence date
2 December 2021
Defence time
09:00
Defence place
Kemicentrum, sal K:B. Join via zoom: https://lu-se.zoom.us/j/62142644089
Opponent
- Stefan Oscarson (Professor)