Detecting the Vulnerable Plaque
Author
Summary, in English
common causes of mortality and morbidity in the world. Previous studies have pointed out inflammation, driven
by inflammatory lipids, as the keystone in the formation of the rupture prone vulnerable plaque. The risk for a
plaque rupture is greatly enhanced in the diabetic population and increased inflammation has been suggested as the
contributing factor. However, the mechanism remains unknown. One of the current greatest challenges in the
atherosclerotic field is to identify subjects with plaques that are vulnerable before the occurrence of a plaque
rupture.
In this thesis we provide evidence that the enzyme lipoprotein associated phospholipase A2 (LpPLA2) and the
lipids lysophosphatidylcholines and sphingolipids (ceramides, lactosylceramides, glucosylceramides) contribute to
the inflammatory process and that they are associated with the vulnerable plaque. This suggests that the synthesis
of these lipids may be possible therapeutic targets. We also show that the inflammatory process in the plaque is
reflected in the peripheral blood, as measured by cytokines, whereas no association was found for soluble
urokinase-type plasminogen activator receptor. The inflammatory cytokines MIP-1β, TNF-α and fractalkine
predict the inflammatory plaque with high sensitivity and specificity, proposing that these inflammatory cytokines
could be used as plasma markers for plaque inflammation. Finally, we provide novel data regarding the biology of
the diabetic plaque. According to our data the inflammatory activity is not increased in the diabetic plaque tissue.
Diabetic plaques contained less stabilizing extracellular matrix proteins (collagen and elastin). Additionally the
plaque levels of the inflammation regulating cytokine IL-10 and the growth factor platelet derived growth factor
as well as the smooth muscle cell stimulating matrix metalloproteinase 2 were significantly decreased in diabetic
plaque tissue. These findings were independent of the presence of symptoms.
In summary, this thesis shows that lipids as LysoPCs and glycosphingolipids are important inducers of plaque
inflammation and may be possible therapeutic targets. In the general population the inflammatory activity is an
important factor for the formation of a vulnerable plaque and this inflammatory process is reflected in blood,
hinting a role for fractalkine, MIP-1β and TNF-α as future biomarkers. In the diabetic population, however, an
impaired tissue repair mechanism seems to be the keystone of plaque vulnerability, rather than inflammation,
emphasizing the need for new therapeutic approaches for diabetics.
Department/s
Publishing year
2013
Language
English
Publication/Series
Lund University Faculty of Medicine Doctoral Dissertation Series
Volume
2013:116
Document type
Dissertation
Publisher
Experimental Cardiovascular Research Unit, Lund University
Topic
- Cardiac and Cardiovascular Systems
Keywords
- Atherosclerosis
- Carotid plaque
- Inflammation
- Diabetes
- LpPLA2
- suPAR
- Cytokines
- Lipids
Status
Published
Research group
- Cardiovascular Research - Immunity and Atherosclerosis
Supervisor
ISBN/ISSN/Other
- ISSN: 1652-8220
- ISBN: 978-91-87449-89-5
Defence date
15 November 2013
Defence time
13:00
Defence place
Föreläsningssalen på Kvinnokliniken, Jan Waldenströmsgata 47, ingång 74, Skånes Universitetssjukhus i Malmö
Opponent
- Gerard Pasterkamp (MD, PhD)