Pseudovirions in the Study of Papilloma- and Polyomaviruses
Author
Summary, in English
Site-directed mutagenesis of the surface loops of the HPV capsid found that these loops were essential for the incorporation of the minor capsid protein L2, the genome encapsidation and proper immunogenicity of the particle.
Pseudovirion-based methods were correlated to presence of viral DNA. The pseudovirion neutralization assays and multiplexed assays using pseudovirions bound to heparin-coated fluorescent beads for 21 HPV and 2 HPyV types were correlated with viral DNA for 16 HPV types and MCPyV. MCPyV specific antibody levels in serum were found to be strongly correlated to the MCPyV viral load in skin. Finally, biobank-based seroepidemiological studies found that MCPyV infection was associated with an increased risk for Merkel cell carcinoma (MCC), in particular among females.
Department/s
- Clinical Microbiology, Malmö
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publishing year
2012
Language
English
Publication/Series
Lund University Faculty of Medicine Doctoral Dissertation Series
Volume
2012:105
Full text
Document type
Dissertation
Publisher
Laboratory Medicine, Medical Microbiology Malmö
Topic
- Microbiology in the medical area
Keywords
- Human papilloma- and polyomaviruses
- pseudovirions
- serology
Status
Published
Research group
- Clinical Microbiology, Malmö
- Institution of Laboratory Medicine, Medical Microbiolgy, Respiratory Pathogen Group
Supervisor
- Joakim Dillner
- Ola Forslund
ISBN/ISSN/Other
- ISSN: 1652-8220
- ISBN: 978-91-87189-68-5
Defence date
21 December 2012
Defence time
09:00
Defence place
Patologiska institutionens föreläsningssal, Jan Waldenströms gata 59, SUS, Malmö
Opponent
- Massimo Tommasino (Dr.)