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The pTalpha promoter and enhancer are direct targets for transactivation by E box-binding proteins.

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Author

Summary, in English

The pre-Talpha (pTalpha[?]) gene encodes a protein that forms the pre-TCR together with the newly expressed TCR beta chain during a defined stage of early T cell development. The restricted expression of this gene has been suggested to depend on a promoter and an enhancer element containing E boxes, which are potential binding sites for basic helix-loop-helix (bHLH) proteins. We here report that increased expression of E47 and E12 mRNA in CD4(--)CD8(--)TCR(--) thymocytes correlates with the initiation of pTalpha[?] transcription. Furthermore, electrophoretic mobility shift assays suggested that E47 binds directly to both the pTalpha[?] promoter and enhancer in vitro. Ectopic expression of E47 in non-lymphoid HeLa cells resultedin a dose- and template-dependent activation of these control elements. In addition to this, ectopic expression of E47 in combination with the ets-protein Pu.1 and[?]/[?]or the Pu.1-interacting protein(Pip-1), resulted in a synergistic effect on the activity of the pTalpha[?] control elements. Thus, we suggest that E47, Pu.1 and Pip-1 are directly involved in the regulation of pTalpha expression inearly T cell development.

Publishing year

2002

Language

English

Pages

911-920

Publication/Series

European Journal of Immunology

Volume

32

Issue

3

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Immunology in the medical area

Keywords

  • Gene Expression Regulation : genetics
  • Hela Cells
  • Genes T-Cell Receptor alpha
  • Hamsters
  • Enhancer Elements (Genetics)
  • Electrophoretic Mobility Shift Assay
  • DNA-Binding Proteins/biosynthesis : genetics : physiology
  • Binding Sites
  • Animal
  • Promoter Regions (Genetics)
  • Protein Binding
  • Protein Precursors : genetics
  • Proto-Oncogene Proteins : genetics : physiology
  • RNA Messenger : genetics
  • Recombinant Fusion Proteins : genetics : metabolism
  • Regulatory Sequences Nucleic Acid
  • Trans-Activation (Genetics)
  • Trans-Activators : genetics : physiology
  • Transfection
  • Transcription Factors : physiology
  • Helix-Loop-Helix Motifs
  • Human
  • Mesocricetus
  • Mice
  • Mice Inbred C57BL

Status

Published

Research group

  • Cancer Immunology, Malmö
  • Immunology

ISBN/ISSN/Other

  • ISSN: 1521-4141