Versatile microchip utilising ultrasonic standing waves
Author
Summary, in English
This paper presents the concept and initial work on a
microfluidic platform for bead-based analysis of
biological sample. The core technology in this project
is ultrasonic manipulation and trapping of particle in
array configurations by means of acoustic forces. The
platform is ultimately aimed for parallel multistep
bioassays performed on biochemically activated
microbeads (or particles) using submicrolitre sample
volumes. A first prototype with three individually
controlled particle trapping sites has been developed
and evaluated. Standing ultrasonic waves were
generated across a microfluidic channel by integrated
PZT ultrasonic microtransducers. Particles in a fluid
passing a transducer were drawn to pressure minima
in the acoustic field, thereby being trapped and
confined laterally over the transducer. It is
anticipated that acoustic trapping using integrated
transducers can be exploited in miniaturised total
chemical analysis systems (µTAS), where e.g.
microbeads with immobilised antibodies can be
trapped in arrays and subjected to minute amounts of
sample followed by a reaction, detected using
fluorescence. Preliminary results indicate that the
platform is capable of handling live cells as well as
microbeads. A first model bioassay with detection of
fluorescein marked avidin binding to trapped biotin
beads has been evaluated.
microfluidic platform for bead-based analysis of
biological sample. The core technology in this project
is ultrasonic manipulation and trapping of particle in
array configurations by means of acoustic forces. The
platform is ultimately aimed for parallel multistep
bioassays performed on biochemically activated
microbeads (or particles) using submicrolitre sample
volumes. A first prototype with three individually
controlled particle trapping sites has been developed
and evaluated. Standing ultrasonic waves were
generated across a microfluidic channel by integrated
PZT ultrasonic microtransducers. Particles in a fluid
passing a transducer were drawn to pressure minima
in the acoustic field, thereby being trapped and
confined laterally over the transducer. It is
anticipated that acoustic trapping using integrated
transducers can be exploited in miniaturised total
chemical analysis systems (µTAS), where e.g.
microbeads with immobilised antibodies can be
trapped in arrays and subjected to minute amounts of
sample followed by a reaction, detected using
fluorescence. Preliminary results indicate that the
platform is capable of handling live cells as well as
microbeads. A first model bioassay with detection of
fluorescein marked avidin binding to trapped biotin
beads has been evaluated.
Department/s
Publishing year
2005
Language
English
Pages
123-124
Publication/Series
IFMBE Proceedings 2005
Document type
Conference paper
Topic
- Medical Engineering
Conference name
13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics
Conference date
2005-06-13 - 2006-06-15
Status
Published