Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target
Author
Summary, in English
A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI IVIS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides.
Publishing year
2006
Language
English
Pages
1071-1081
Publication/Series
Journal of Proteome Research
Volume
5
Issue
5
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Medical Engineering
- Analytical Chemistry
Keywords
- proteomics
- spectrometry
- mass
- SPE
- solid-phase extraction
- solid-phase microextraction
- SPME
- integrated selective enrichment target
- ISET
- MALDI MS
Status
Published
ISBN/ISSN/Other
- ISSN: 1535-3893