The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Non-specific effects of leukotriene synthesis inhibitors on HeLa cell physiology

På svenska

Author

  • A Sabirsh
  • Jesper Bristulf
  • Ulf Karlsson
  • Christer Owman
  • JZ Haeggstrom

Summary, in English

We examined the effects of various leukotriene synthesis inhibitors on calcium signalling in HeLa cells, before and after transfection with BLT1. All of the inhibitors studied were found to reduce increases in intracellular calcium concentration induced by BLT1, but also by an ionophore or activation of various G-protein coupled receptors, regardless of BLT1 expression. In order to explore the mechanism of these apparently general effects we examined HeLa cell expression of leukotriene receptors and biosynthetic enzymes and found that the genes for key leukotriene synthesis enzymes and all of the leukotriene receptors were not expressed. Leukotrienes are involved in the pathology of a variety of cancers, and for HeLa cells leukotrienes have been reported to be important for aspects of the carcinogenic phenotype. We find that leukotriene synthesis inhibitors have non-specific effects, so careful controls are necessary to avoid interpreting non-specific effects as evidence for leukotriene involvement.

Department/s

Publishing year

2005

Language

English

Pages

431-440

Publication/Series

Prostaglandins, Leukotrienes and Essential Fatty Acids

Volume

73

Issue

6

Document type

Journal article

Publisher

Elsevier

Topic

  • Neurosciences

Status

Published

Research group

  • Drug Target Discovery

ISBN/ISSN/Other

  • ISSN: 0952-3278