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Pituitary Homeobox 2 Regulates Adrenal4 Binding Protein/Steroidogenic Factor-1 Gene Transcription in the Pituitary Gonadotrope through Interaction with the Intronic Enhancer

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Author

  • Yuichi Shima
  • Mohamad Zubair
  • Tomoko Komatsu
  • Sanae Oka
  • Chikako Yokoyama
  • Taro Tachibana
  • Tord Hjalt
  • Jacques Drouin
  • Ken-Ichirou Morohashi

Summary, in English

Ad4BP/SF-1 (Adrenal4 Binding Protein/Steroidogenic Factor-1 (NR5A1)) is a factor important for animal reproduction and endocrine regulation, and its expression is tightly regulated in the gonad, adrenal gland, ventromedial hypothalamic nucleus (VMH), and pituitary gonadotrope. Despite its functional significance in the pituitary, the mechanisms underlying pituitary-specific expression of the gene remain to be uncovered. In this study, we demonstrate by transgenic mouse assays that the pituitary gonadotrope-specific enhancer is localized within the 6th intron of the gene. Functionally, the enhancer recapitulates endogenous Ad4BP/SF-1 expression in the fetal Rathke's pouch to the adult pituitary gonadotrope. Structurally, the enhancer consists of several elements conserved among animal species. Mutational analyses confirmed the significance of these elements for the enhancer function. One of these elements was able to interact both in vitro and in vivo with Pitx2, demonstrating that Pitx2 regulates Ad4BP/SF-1 gene transcription in the pituitary gonadotrope via interaction with the gonadotrope-specific enhancer.

Publishing year

2008

Language

English

Pages

1633-1646

Publication/Series

Molecular Endocrinology

Volume

22

Issue

7

Document type

Journal article

Publisher

The Endocrine Society

Topic

  • Endocrinology and Diabetes

Status

Published

ISBN/ISSN/Other

  • ISSN: 0888-8809