The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Autonomous protein sample processing on-chip using solid-phase microextraction, capillary force pumping, and microdispensing

På svenska

Author

Summary, in English

A capillary force filling microsystem consisting of a chip-integrated solid-phase microextraction (SMEC) array and a microdispenser for sample purification and trace enrichment of peptides is described. The microextraction array was loaded with solid-phase media (50 mum Poros R2 beads) for purification and enrichment of proteomic samples. Samples bound to the SMEC were eluted in a volume of 200 nL. A piezoelectric microdispenser was docked to the array and the samples bound to the SMEC were eluted in a volume of 200 nL using capillary forces. The purified and enriched samples were dispensed onto the matrix-assisted laser desorption/ionization (MALDI) target, providing quality data from samples in the picomolar range. The nanoproteomic platform was compared to corresponding commercial preparation protocols, showing higher mass spectrometry (MS) signal intensities for peptides generated from an alpha-casein digest. The platform was also elvaluated with regards to two-dimensional (2-D) gel-derived protein digests from both fibroblast and epithelial target cells.

Publishing year

2004

Language

English

Pages

3778-3787

Publication/Series

Electrophoresis

Volume

25

Issue

21-22

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Medical Engineering
  • Analytical Chemistry

Keywords

  • microextraction
  • miniaturization
  • mass spectrometry
  • solid-phase
  • proteomics

Status

Published

ISBN/ISSN/Other

  • ISSN: 0173-0835