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Thylakoid enriched powder product Appenthyl is resistant against gastrointestinal digestion and keeps on inhibiting lipolysis

Author:
  • Manjuma Asheka
Publishing year: 2014
Language: English
Document type: Student publication for Master's degree (two years)

Summary

In earlier studies it was revealed that thylakoid membranes inhibit activity of pancreatic lipase/colipase. More studies have shown that these membranes induce satiety hormone CCK and reduce hunger hormone of rats and human. Appethyl® used in this project is processed from spinach leaves in such a way that it reserves most amounts of thylakoid membranes. The results, in this project, shows that Appethyl® can inhibit the activity of lipase/colipase after digestive treatment. Separate reactions indicate highest inhibition capacity of Appethyl® after pepsin and gastric juice digestion. The positive thing is that, Appethyl® shows inhibition capacity on lipase/colipase even after treatment with pepsin, trypsin, pepsin/ trypsin, gastric juice (GJ), pancreatic juice(PJ), gastric/pancreatic juice. Treatment with GJ followed by long time in PJ also does not affect the thylakoid membranes present in Appethyl®. This conclusion was based on SDS-PAGE electrophoresis gel picture with Appethyl® incubated 45 minutes in GJ and 0-6 hours in PJ. This is also supported by the result of inhibition capacity by Appethyl® as 36%- 31% after GJ /PJ treatment. SDS-PAGE gel treated and untreated Appethyl® showed that there were protein bands at the MW position for LCH II and Rubisco large chain and Rubisco small chain.

Keywords

  • Chemistry
  • biokemi
  • biochemistry

Other

  • Per-Åke Albertsson
  • Sinan Cem Emek