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Positively Regulated Glycerol/G3P-Dependent Bacillus subtilis Gene Expression System Based on Anti-Termination.

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Author

Summary, in English

Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive control, does not involve a DNA-binding protein, and that glycerol, a cheap and stable compound, can be used as inducer of gene expression.

Publishing year

2009

Language

English

Pages

61-70

Publication/Series

Journal of Molecular Microbiology and Biotechnology

Volume

17

Document type

Journal article

Publisher

Karger

Topic

  • Microbiology

Keywords

  • Anti-termination
  • glp
  • Glycerol inducible
  • Bacillus subtilis

Status

Published

ISBN/ISSN/Other

  • ISSN: 1464-1801