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A critical role for Ebf1 and Ebf2 in the adipogenic transcriptional cascade.

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Author

Summary, in English

The Ebf (O/E) family of helix-loop-helix transcription factors plays a significant role in B lymphocyte and neuronal development. The three primary members of this family, Ebf1, 2, and 3, are all expressed in adipocytes, and Ebf1 promotes adipogenesis when overexpressed in NIH 3T3 fibroblasts. Here we report that these three proteins have adipogenic potential in multiple cellular models and that peroxisome proliferator-activated receptor gamma (PPAR gamma) is required for this effect, at least in part due to direct activation of the PPAR gamma 1 promoter by Ebf1. Ebf1 also directly binds to and activates the C/EBP alpha promoter, which exerts positive feedback on C/EBP delta expression. Despite this, C/EBP alpha is dispensable for the adipogenic action of Ebf proteins. Ebf1 itself is induced by C/EBP beta and delta, which bind and activate its promoter. Reduction of Ebf1 and Ebf2 proteins by specific short hairpin RNA blocks differentiation of 3T3-L1 cells, suggesting a critical role for these factors and the absence of functional redundancy between members of this family. Altogether, these data place Ebf1 within the known transcriptional cascade of adipogenesis and suggest critical roles for Ebf1 and Ebf2.

Department/s

Publishing year

2007

Language

English

Pages

743-757

Publication/Series

Molecular and Cellular Biology

Volume

27

Issue

Oct 23

Document type

Journal article

Publisher

American Society for Microbiology

Topic

  • Cell and Molecular Biology

Status

Published

ISBN/ISSN/Other

  • ISSN: 0270-7306