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Effects of ultraviolet-radiation on viability of isolated beta-vulgaris and hordeum-vulgare protoplasts

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Author

Summary, in English

Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B radiation decreased the intensity of DLE by 40% without appreciably affecting the decay rate. Longer treatment times did not give a proportional effect so that even after 60 min of UV-B the inhibition did not exceed 60%. This suggested that although the enzyme system responsible for FDA hydrolysis may be partially inactivated (viability was 75-80% as compared with 90% in the control), the UV-B did not penetrate the innermost parts of the chloroplasts, but left some thylakoids undamaged.

Publishing year

1982

Language

English

Pages

297-306

Publication/Series

Zeitschrift für Pflanzenphysiologie

Volume

105

Issue

4

Document type

Journal article

Publisher

Gustav Fischer Verlag

Topic

  • Biological Sciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 0044-328X