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Novel antibody specificities targeting glycoprotein B of cytomegalovirus identified by molecular library technology

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Author

  • Fredrika Carlsson
  • Jonas Persson
  • Emmanuel Moreau
  • Marie-Hélène Côté
  • Alain Lamarre
  • Mats Ohlin

Summary, in English

Antibodies provide some protection against cytomegalovirus-mediated disease. All aspects of antibody recognition of important viral antigens are however not fully appreciated. Glycoprotein B (gB), a key protein in the viral membrane, participates in viral infection and it is a component of prototype vaccines. By using combinatorial antibody library and selection technology, novel antibody specificities targeting gB have now been isolated. We define a monoclonal antibody fragment able to recognize site I of antigenic domain (AD) 2, a poorly immunogenic epitope targeted by potent virus-neutralizing antibodies, in a way that is different from the binding of antibodies induced by infection but similar to those induced by vaccination. We also describe the existence of a novel epitope overlapping site I of AD-2 and AD-1, the immunodominant epitope of gB. These specificities, derived by molecular engineering, will be useful for the future assessment of humoral immune responses against this opportunistic viral infection.

Publishing year

2009

Language

English

Pages

429-436

Publication/Series

New Biotechnology

Volume

25

Issue

6

Document type

Journal article

Publisher

Elsevier

Topic

  • Immunology in the medical area

Status

Published

ISBN/ISSN/Other

  • ISSN: 1876-4347