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Versatile microchip utilising ultrasonic manipulation of microparticles

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Author

Summary, in English

This paper presents the concept and initial work on a microfluidic platform for bead-based analysis of biological sample. The core technology in this project is ultrasonic manipulation and trapping of particle in array configurations by means of acoustic forces. The platform is ultimately aimed for parallel multistep bioassays performed on biochemically activated microbeads (or particles) using submicrolitre sample volumes. A first prototype with three individually controlled particle trapping sites has been developed and evaluated. Standing ultrasonic waves were generated across a microfluidic channel by integrated PZT ultrasonic microtransducers. Particles in a fluid passing a transducer were drawn to pressure minima in the acoustic field, thereby being trapped and confined laterally over the transducer. It is anticipated that acoustic trapping using integrated transducers can be exploited in miniaturised total chemical analysis systems (μTAS), where e.g. microbeads with immobilised antibodies can be trapped in arrays and subjected to minute amounts of sample followed by a reaction, detected using fluorescence. Preliminary results indicate that the platform is capable of handling live cells as well as microbeads. A first model bioassay with detection of fluorescein marked avidin binding to trapped biotin beads has been evaluated

Publishing year

2005

Language

English

Pages

123-124

Publication/Series

Proceedings of the International Federation for Medical & Biomedical Engineering. 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics

Document type

Conference paper

Publisher

Int. Federation for Medical and Biological Eng

Topic

  • Medical Engineering

Keywords

  • acoustic field
  • integrated PZT ultrasonic microtransducer
  • microfluidic channel
  • standing ultrasonic wave
  • submicrolitre sample volume
  • biochemically activated microbead
  • parallel multistep bioassay
  • biological sample
  • microparticle
  • versatile microchip
  • ultrasonic manipulation
  • acoustic trapping
  • chemical analysis system
  • immobilised antibody
  • fluorescein marked avidin binding
  • biotin bead

Conference name

Proceedings of the International Federation for Medical & Biomedical Engineering. 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics

Conference date

2005-06-13 - 2005-06-17

Conference place

Umea, Sweden

Status

Published

ISBN/ISSN/Other

  • ISBN: 91-7305-910-2