In situ poly(ethylene imine) coating of hollow fiber membranes used for microdialysis sampling

Summary, in English

A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high-molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyrne complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least affected the performance of the polysulfone membranes. Conversion of maltoheptaose to maltotriose and maltose was increased reproducibly (within a 5 % relative standard deviation) by 50 % for modified membranes compared to the native hollow fiber membranes. The results demonstrate the potential to effectively modify membranes already fitted on a microdialysis probe. Such a procedure can be modified and employed to either promote or reduce membrane-protein interaction for hollow fibers used as microdialysis sampling units or other similar membrane applications.

• Author: N Torto; M Ohlrogge; Lo Gorton; J M Van Alstine; Thomas Laurell; György Marko-Varga
• Department/s: Centre for Analysis and Synthesis; Department of Biomedical Engineering
• Publishing year: 2004
• Language: English
• Pages: 879-888
• Publication/Series: Pure and Applied Chemistry
• Volume: 76
• Issue: 4
• Links: http://dx.doi.org/10.1351/pac200476040879
• Document type: Journal article
• Publisher: IUPAC
• Topic: Analytical Chemistry; Medical Engineering
• Status: Published
• ISBN/ISSN/Other: ISSN: 0033-4545