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Generic workflow for quality assessment of quantitative label-free LC-MS analysis

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Author

Summary, in English

As high resolution instruments are becoming standard in proteomics laboratories, label-free quantification using precursor measurements is becoming a viable option, and is consequently rapidly gaining popularity. Several software solutions have been presented for label-free analysis, but to our knowledge no conclusive studies regarding the sensitivity and reliability of each step of the analysis procedure has been described. Here we use real complex samples to assess the reliability of label-free quantification using four different software solutions. A generic approach to quality test quantitative label-free LC-MS is introduced. Measures for evaluation are defined for feature detection, alignment and quantification. All steps of the analysis could be considered adequately performed by the utilized software solutions, although differences and possibilities for improvement could be identified. The described method provides an effective testing procedure, which can help the user to quickly pinpoint where in the workflow changes are needed.

Publishing year

2011

Language

English

Pages

1114-1124

Publication/Series

Proteomics

Volume

11

Issue

6

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Immunology in the medical area

Keywords

  • quality control
  • LC-MS
  • label-free quantification

Status

Published

ISBN/ISSN/Other

  • ISSN: 1615-9861