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140 mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry.

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Author

Summary, in English

Calmodulin is an essential Ca2+-binding protein that binds to a variety of targets that carry out critical signaling functions. We describe the proteomic characterization of mouse brain Ca2+-calmodulin-binding proteins that were purified using calmodulin affinity chromatography. Proteins in the eluates from four different affinity chromatography experiments were identified by 1-DE and in-gel digestion followed by LC-MS/MS. Parallel experiments were performed using two related control-proteins belonging to the EF-hand family. After comparing the results from the different experiments, we were able to exclude a significant number of proteins suspected to bind in a nonspecific manner. A total of 140 putative Ca2+-calmodulin-binding proteins were identified of which 87 proteins contained calmodulin-binding motifs. Among the 87 proteins that contained calmodulin-binding motifs, 48 proteins have not previously been shown to interact with calmodulin and 39 proteins were known calmodulin-binding proteins. Many proteins with ill-defined functions were identified as well as a number of proteins that at the time of the analysis were described only as ORFs. This study provides a functional framework for studies on these previously uncharacterized proteins.

Publishing year

2006

Language

English

Pages

669-687

Publication/Series

Journal of Proteome Research

Volume

5

Issue

3

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Topic

  • Basic Medicine

Keywords

  • calcium
  • protein-protein interactions
  • calmodulin
  • proteomics
  • brain

Status

Published

Research group

  • Celiac Disease and Diabetes Unit

ISBN/ISSN/Other

  • ISSN: 1535-3893