Hydrolysis of maltoheptaose in flow through silicon wafer microreactors containing immobilised alpha-amylase and glycoamylase
Author
Summary, in English
In this study,a silicon micro immobilised enzyme reactor (mu IMER) has been applied for hydrolysis of maltoheptaose as a model maltodextrin and starch using immobilised otamylase (from Aspergillus oryzae) and glycoamylase (from Aspergillus niger). The influence of several parameters was investigated such as immobilisation chemistry, buffer constituents, pH, temperature, flow rate and substrate concentration. The conversion efficiency profile of the substrate was measured and the long-term stability of the reactor was tested. For separation and detection of the formed hydrolysis products, high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used. The results show that the mu IMERs can also be used for hydrolysis of starch and also additionally be connected directly on-line with, e.g., liquid chromatography, making it possible to perform on-line characterisation and analysis of starch hydrolysis products.
Publishing year
2006
Language
English
Pages
231-242
Publication/Series
Stärke
Volume
58
Issue
5
Document type
Journal article
Publisher
John Wiley & Sons Inc.
Topic
- Analytical Chemistry
- Medical Engineering
Keywords
- glycoamylase
- immobilised enzyme silicon reactor
- enzymatic degradation
- alpha-amylase
Status
Published
ISBN/ISSN/Other
- ISSN: 0038-9056