Mesenchymal stromal cells from primary osteosarcoma are non-malignant and strikingly similar to their bone marrow counterparts.

Summary, in English

Mesenchymal stromal cells (MSC) are multipotent cells that can be isolated from a number of human tissues. In cancer, MSC have been implicated with tumor growth, invasion, metastasis, drug resistance and were even suggested as possible tumor-initiating cells in osteosarcoma (OS). However, MSC from OS and their possible tumor origin have not yet been thoroughly investigated. Therefore, primary OS mesenchymal progenitors and OS-derived MSC were studied. OS samples contained very high frequencies of mesenchymal progenitor cells as measured by the CFU-F assay (median: 1,117 colonies per 10(5) cells, range: 133 - 3,000, n=6). This is considerably higher compared to other human tissues such as normal bone marrow (1.3 ± 0.2 colonies per 10(5) cells, n=8). OS-derived MSC (OS-MSC) showed normal MSC morphology and expressed the typical MSC surface marker profile (CD105/CD73/CD90/CD44/HLA-classI/CD166 positive, CD45/CD34/CD14/CD19/HLA-DR/CD31 negative). Furthermore, all OS-MSC samples could be differentiated into the osteogenic lineage, and all but one sample into adipocytes and chondrocytes. Genetic analysis of OS-MSC as well as OS-derived spheres showed no tumor-related chromosomal aberrations. OS-MSC expression of markers related to tumor-associated fibroblasts (fibroblast surface protein, alpha-smooth muscle actin, vimentin) was comparable to bone marrow MSC and OS-MSC growth was considerably affected by tyrosine kinase inhibitors. Taken together, our results demonstrate that normal, non-malignant mesenchymal stroma cells are isolated from OS when MSC culture techniques are applied. OS-MSC represent a major constituent of the tumor microenvironment, and they share many properties with bone marrow-derived MSC. © 2010 UICC.

• Author: Jan Claas Brune; Ariane Tormin; Maria C Johansson; Pehr Rissler; Otte Brosjö; Richard Löfvenberg; Fredrik Vult von Steyern; Fredrik Mertens; Anders Rydholm; Stefan Scheding
• Department/s: Stem Cell Center; Breastcancer-genetics; Tumor microenvironment; Orthopaedics (Lund); Division of Clinical Genetics; StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
• Publishing year: 2011
• Language: English
• Pages: 319-330
• Publication/Series: International Journal of Cancer
• Volume: 129
• Links: http://www.ncbi.nlm.nih.gov/pubmed/20878957?dopt=Abstract; http://dx.doi.org/10.1002/ijc.25697
• Document type: Journal article
• Publisher: John Wiley & Sons Inc.
• Topic: Cancer and Oncology
• Keywords: mesenchymal stromal cells; osteosarcoma; osteogenic sarcoma; cancer associated fibroblasts; tumor stroma
• Status: Published
• ISBN/ISSN/Other: ISSN: 0020-7136