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Enzyme conformational alterations detected by partition column chromatography

På svenska

Author

  • Christer Wingren
  • Ulla-Britt Hansson

Summary, in English

In this paper, we demonstrate the ability of liquid-liquid partition chromatography (LLPC) to detect conformational alterations occurring in well-characterized enzymes. The conformational changes induced in dehydrogenases such as alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenases (LDH) and malate dehydrogenase (MDH) upon binding of ligand(s) were detectable by LLPC. The ligand-dependent equilibrium between two forms of citrate synthase (CS), glutamate-oxaloacetate transaminase (GOT), hexokinase (HK) and 3-phosphoglycerate kinase (PGK) could also be demonstrated. Furthermore, different conformational forms of some of the apoenzymes could also be detected and separated by LLPC. The results obtained here are discussed in relation to those obtained by other methods.

Publishing year

1996

Language

English

Pages

113-122

Publication/Series

Journal of Chromatography. B

Volume

680

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • Partition column chromatography
  • Conformational changes
  • Enzymes

Status

Published

ISBN/ISSN/Other

  • ISSN: 1572-6495