Lipopolysaccharide induces cell death in cultured porcine myenteric neurons.
Author
Summary, in English
Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without α-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in α-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous presence of α-ketoglutarate or L-NAME. Either SNAP or α-ketoglutarate influenced neuronal survival. Culturing, particularly in the presence of LPS, markedly increased the proportion of VIP-immunoreactive neurons; NOS-immunoreactive neurons were unchanged. The reported LPS-induced neurotoxicity indicates loss of enteric neurons as a consequence of intestinal inflammation.
Department/s
- Functional zoology
- Neurogastroenterology
Publishing year
2005
Language
English
Pages
1661-1668
Publication/Series
Digestive Diseases and Sciences
Volume
50
Issue
9
Full text
- Available as PDF - 258 kB
- Download statistics
Links
Document type
Journal article
Publisher
Springer
Topic
- Gastroenterology and Hepatology
Keywords
- Myenteric Plexus: cytology
- Animals
- Cell Culture Techniques
- Cell Death
- Cell Survival
- Female
- Immunohistochemistry
- Inflammation
- Intestinal Diseases: etiology
- Intestinal Diseases: immunology
- Intestinal Diseases: microbiology
- Lipopolysaccharides: pharmacology
- Male
- Myenteric Plexus: pathology
- Neurons: drug effects
- Neurons: physiology
- Neurotransmitters: biosynthesis
- Research Support
- Non-U.S. Gov't
- Swine
Status
Published
Research group
- Neurogastroenterology
ISBN/ISSN/Other
- ISSN: 1573-2568