Activation of extracellular-signal-regulated kinase-1/2 precedes and is required for injury-induced Schwann cell proliferation.

Summary, in English

Activation of extracellular-signal-regulated kinase-1/2 (Erkl/2) by phosphorylation to p-Erkl/2, and proliferation of Schwann cells were investigated in the rat sciatic nerve by immunohistochemistry. Axotomy in vivo and culturing of nerve segments in vitro resulted in a rapid (30 min) increase of p-Erkl/2 in Schwann cells with peaks at 2 and 24 h. Proliferation measured by bromodeoxy uridine incorporation and immunostaining in vivo and in vitro 48 h after axotomy showed an increase in Schwann cell proliferation at the sites of Erkl/2 activation. The Erkl/2 inhibitor U0126 inhibited both the increase in p-Erkl/2 and the bromodeoxy uridine incorporation. We suggest that an increase in p-Erkl/2 is required for nerve injury-induced proliferation of Schwann cells.

• Author: Lisa Mårtensson; Per Gustavsson; Lars B Dahlin; Martin Kanje
• Department/s: Functional zoology
• Publishing year: 2007
• Language: English
• Pages: 957-961
• Publication/Series: NeuroReport
• Volume: 18
• Issue: 10
• Links: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17558277&dopt=Abstract
• Document type: Journal article
• Publisher: Lippincott Williams & Wilkins
• Topic: Neurosciences
• Keywords: Erk map kinases; cellular proliferation; nerve injury; regeneration; phosphorylation; Schwann cells; nerve
• Status: Published
• Project: Nerve regeneration - signal transduktion mechanisms, timing and alternatives to nerve grafts
• ISBN/ISSN/Other: ISSN: 1473-558X