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Microbial biosensor for the analysis of 2,4-dichlorophenol

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Author

  • Jongjit Jantra
  • Hamid Zilouei
  • Jing Liu
  • Benoit Guieysse
  • P Thavarungkul
  • P Kanatharana
  • Bo Mattiasson

Summary, in English

A flow injection cell-based biosensor was constructed for 2,4-dichlorophenol (DCP) analysis by using a Clark-type oxygen electrode as a transducer. A mixed bacterial culture capable to biodegrade DCP was immobilized between a Teflon membrane and a dialysis membrane and attached to the oxygen electrode. Optimization of the flow rate, the injection volume, the carrier buffer concentration, and pH was carried out. Under optimum conditions (100 mM phosphate buffer, pH 7.50; flow rate 0.10 mL min(-1); sample volume 100 mL), the sensor response was linear between 0.01 and 0.30 mM DCP. The detection limit was 0.02 mM DCP, and the sensor was quite stable during 5 days of operation.

Department/s

  • Biotechnology

Publishing year

2005

Language

English

Pages

1071-1083

Publication/Series

Analytical Letters

Volume

38

Issue

7

Document type

Journal article

Publisher

Taylor & Francis

Topic

  • Industrial Biotechnology

Status

Published

ISBN/ISSN/Other

  • ISSN: 0003-2719