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Flow-Through-Mediated Surface Immobilization of Sub-Micrometre Particles in Monolithic Cryogels

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Author

Summary, in English

Particles with functional groups on their surface were immobilized on premade cryogels through covalent bonding to maximize the accessibility of the target molecules to the surface of the particles. The physical properties of the composite cryogel were characterized by SEM, texture analysis and nitrogen adsorption. Different key factors affecting immobilization were also investigated, e.g., recirculation time and the number of functional groups on the premade cryogels. It was found that the physical characteristics of the composite cryogels prepared in this way were very similar to those of the primary matrices, while their binding capacity increased in comparison with conventionally prepared composite cryogels. The binding capacities of two different types of composite cryogel were evaluated by studying the adsorption of small molecules (copper ions and Orange G) and a large molecule (thermostable His-tagged exoglucanase).

Department/s

Publishing year

2014

Language

English

Pages

631-638

Publication/Series

Macromolecular Materials and Engineering

Volume

299

Issue

5

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Industrial Biotechnology

Keywords

  • composite
  • covalent interaction
  • flow-through immobilization
  • macroporous hydrogel
  • post modification

Status

Published

ISBN/ISSN/Other

  • ISSN: 1438-7492