Purification of IgD from human serum - A novel application of recombinant M. catarrhalis IgD-binding protein (MID).
Author
Summary, in English
Moraxella catarrhalis IgD-binding protein (MID) is a multimeric outer membrane protein belonging to the family of autotransporters. The IgD-binding domain of MID is located between amino acids MID 962-1200 and binds to amino acids 198-224 of the IgD C(H)1 region. In the present study, we describe a method to purify IgD from serum with high levels of IgD using a two-step affinity chromatography process. The first step involves depletion of MID-specific antibodies of all classes from serum using the non-IgD-binding fragment MID1000-1200. This step is followed by selective capture of IgD with MID962-1200. Furthermore, we demonstrate that the eluted IgD is pure, intact and functional for use in downstream applications. Our approach reduces the non-specificity commonly associated with lectin-based IgD purification regimes that rely on glycosylation of the IgD molecule. (c) 2006 Elsevier B.V. All rights reserved.
Department/s
Publishing year
2006
Language
English
Pages
31-37
Publication/Series
Journal of Immunological Methods
Volume
317
Links
Document type
Journal article
Publisher
Elsevier
Topic
- Immunology in the medical area
Keywords
- purification
- immunoglobulin D
- Moraxella catarrhalis
- MID
Status
Published
Research group
- Clinical Microbiology, Malmö
ISBN/ISSN/Other
- ISSN: 1872-7905