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Galectin-3, a marker for vacuole lysis by invasive pathogens

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Author

  • Irit Paz
  • Martin Sachse
  • Nicolas Dupont
  • Joelle Mounier
  • Cecilia Cederfur
  • Jost Enninga
  • Hakon Leffler
  • Francoise Poirier
  • Marie-Christine Prevost
  • Frank Lafont
  • Philippe Sansonetti
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Summary, in English

P>Shigella bacteria invade macrophages and epithelial cells and following internalization lyse the phagosome and escape to the cytoplasm. Galectin-3, an abundant protein in macrophages and epithelial cells, belongs to a family of beta-galactoside-binding proteins, the galectins, with many proposed functions in immune response, development, differentiation, cancer and infection. Galectins are synthesized as cytosolic proteins and following non-classical secretion bind extracellular beta-galactosides. Here we analysed the localization of galectin-3 following entry of Shigella into the cytosol and detected a striking phenomenon. Very shortly after bacterial invasion, intracellular galectin-3 accumulated in structures in vicinity to internalized bacteria. By using immuno-electron microscopy analysis we identified galectin-3 in membranes localized in the phagosome and in tubules and vesicles that derive from the endocytic pathway. We also demonstrated that the binding of galectin-3 to host N-acetyllactosamine-containing glycans, was required for forming the structures. Accumulation of the structures was a type three secretion system-dependent process. More specifically, existence of structures was strictly dependent upon lysis of the phagocytic vacuole and could be shown also by Gram-positive Listeria and Salmonella sifA mutant. We suggest that galectin-3-containing structures may serve as a potential novel tool to spot vacuole lysis.

Publishing year

2010

Language

English

Pages

530-544

Publication/Series

Cellular Microbiology

Volume

12

Issue

4

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Microbiology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1462-5814