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Capture of bacteriocins directly from non-clarified fermentation broth using macroporous monolithic cryogels with phenyl ligands

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Author

Summary, in English

The bacteriocin, sakacin P was produced by the bacteriocin-producing strain Lactobacillus sakei CCUG 42687 at 20 degrees C without pH control. The bacteriocin was captured directly from the fermentation broth using macroporous octyl- and phenyl-monolith columns. The large size of the interconnected macropores (up to 100 mu m) in the macroporous monolith allowed for direct fermentation broth processing with no clarification needed. Screening for optimal bacteriocin binding demonstrated that at pH 6.2 about 80% of the bacteriocin activity could be recovered with a purification factor of 150-160 in the cell-free eluate. Capture of bacteriocins from the fermentation broth using macroporous monoliths in the 96-well plate format presents a promising approach for rapid analytical isolation of bacteriocins from numerous samples.

Department/s

Publishing year

2007

Language

English

Pages

786-793

Publication/Series

Enzyme and Microbial Technology

Volume

40

Issue

4

Document type

Journal article

Publisher

Elsevier

Topic

  • Industrial Biotechnology

Keywords

  • cryogel in microtiter plate
  • macroporous monolith
  • bacteriocin
  • integrated product adsorption

Status

Published

ISBN/ISSN/Other

  • ISSN: 0141-0229