The adhesion receptor CD-31 can be primed to rapidly adjust the neutrophil cytoskeleton.
Author
Summary, in English
The adhesion receptor CD-31 is expressed on neutrophils and endothelial cells and participates in transendothelial migration of neutrophils. Although necessary, information on CD-31-induced signaling and its influence on the shape-forming actin network is scarce. Here, we found that antibody engagement of CD-31 on suspended neutrophils triggered a prompt intracellular Ca(2+) signal, providing the cells had been primed with a chemotactic factor. Inhibition of Src-tyrosine kinases blocked this Ca(2+) signal, but not a fMet-Leu-Phe-induced Ca(2+) signal. Despite the ability of fMet-Leu-Phe to activate Src-tyrosine kinases, it did not per se induce tyrosine phosphorylation of CD-31. However, fMet-Leu-Phe did enable such a phosphorylation following an antibody-induced engagement of CD-31. This clustering also triggered a Ca(2+)-dependent depolymerization of actin and, surprisingly enough, a simultaneous polymerization. The ability of CD-31 to signal dynamic alterations in the cytoskeleton, particularly the Ca(2+)-induced actin depolymerization, further explains how neutrophils can squeeze themselves out between adjacent endothelial cells.
Department/s
Publishing year
2002
Language
English
Pages
1092-1097
Publication/Series
Biochemical and Biophysical Research Communications
Volume
292
Issue
4
Links
Document type
Journal article
Publisher
Elsevier
Topic
- Biological Sciences
Keywords
- CD31 : drug effects
- Antigens
- Antibodies : pharmacology
- Actins : metabolism
- Cytoskeleton : metabolism
- Enzyme Inhibitors : pharmacology
- Human
- Intracellular Fluid : metabolism
- N-Formylmethionine Leucyl-Phenylalanine : pharmacology
- Neutrophils : cytology
- Neutrophils : metabolism
- Phosphorylation : drug effects
- Signal Transduction : drug effects
- Signal Transduction : physiology
- Support
- Non-U.S. Gov't
- src-Family Kinases : antagonists & inhibitors
- CD31 : metabolism
- Calcium : metabolism
- Calcium Signaling : drug effects
- Cell Adhesion : physiology
- Cell Movement : physiology
- Chemotactic Factors : pharmacology
Status
Published
Research group
- Experimental Pathology, Malmö
ISBN/ISSN/Other
- ISSN: 1090-2104