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Female pig urethral tone is dependent on Rho guanosine triphosphatases and Rho-associated kinase.

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Author

Summary, in English

PURPOSE: Circular smooth muscle of the urethra generates spontaneous myogenic tone of relevance for the maintenance of continence. We tested if Rho guanosine triphosphatases (GTPases) and Rho-associated kinase (ROK) are involved in the generation of urethral tone. MATERIALS AND METHODS: Small circular strips of female pig urethra were dissected out and mounted for recording isometric force. The effect of pharmacological agents known to modulate the activity of Rho GTPases or ROK was examined. The intracellular calcium concentration was measured using fura-2. RESULTS: Urethral tone was abolished by removing extracellular calcium or by adding the calcium antagonist felodipine. The decrease in force was closely related to a decrease in intracellular calcium concentration, indicating that tone depends on membrane associated mechanisms. Toxin B, which inactivates Rho GTPases, and Y 27632, which inhibits ROK, completely abolished tone in the female pig urethra. The latter effect occurred without any change in the intracellular calcium concentration. CONCLUSIONS: The results suggests that urethral tone depends on activity in G-protein coupled pathways and inhibition of this activity is sufficient for urethral tone relaxation. Thus, to our knowledge a new pathway in the generation of urethral tone, which might be acted on by autonomic nerves during micturition, has been identified.

Publishing year

2004

Language

English

Pages

1955-1958

Publication/Series

Journal of Urology

Volume

171

Issue

5

Document type

Journal article

Publisher

Lippincott Williams & Wilkins

Topic

  • Urology and Nephrology

Keywords

  • urethra
  • swine
  • muscle
  • smooth
  • rho-associated kinase
  • GTP-binding proteins

Status

Published

Research group

  • Vascular Physiology
  • Cellular Biomechanics

ISBN/ISSN/Other

  • ISSN: 1527-3792