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Multimeric Lactoside "Click Clusters" as Tools to Investigate the Effect of Linker Length in Specific Interactions with Peanut Lectin, Galectin-1, and-3

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Author

  • Sebastien G. Gouin
  • Jose Manuel Garcia Fernandez
  • Enguerran Vanquelef
  • Francois-Yves Dupradeau
  • Emma Salomonsson
  • Hakon Leffler
  • Mariano Ortega-Munoz
  • Ulf Nilsson
  • Jose Kovensky

Summary, in English

Multimeric lactosides based on carbohydrate scaffolds with valencies ranging from 1 to 4 and different linker lengths were synthesized by a copper-catalyzed azide-alkyne cycloaddition (CuAAC). The binding affinities and crosslinking abilities of the new "click clusters" toward biologically relevant galectins (gal-1, gal-3) and peanut lectin were evaluated by fluorescent polarization assay (FPA) and enzyme-linked lectin assay (ELLA), respectively. FPA indicated that the binding affinities of the synthetic multilactosides towards the galectins increased proportionally with their lactosyl content, without significant differences due to the spacer length. ELLA evidenced a modest cluster effect for the multivalent conjugates, with a relative potency per lactoside ranging from 2.1 to 3.2. Nearly identical binding affinities were recorded for derivatives differing in the length of the linkers, in agreement with the FPA data. These results demonstrate that this parameter does not significantly influence the recognition process when interactions occur at a single lectin site. Molecular dynamics revealed that glycoconjugates adopt a pseudoglobular structure with a random localization of the lactoside residues. These spatial distributions were observed irrespective of the linker length; this explains the virtually equal affinities recorded by ELLA. In contrast, two-site "sandwich" ELLA clearly revealed that multivalent derivatives bearing the longest spacers were more efficient for crosslinking lectins. Intrinsic affinities, devoid of aggregation effects, and crosslinking capabilities are, therefore, not directly related phenomena that must be taking into consideration in neoglycoconjugate design for specific applications.

Publishing year

2010

Language

English

Pages

1430-1442

Publication/Series

ChemBioChem

Volume

11

Issue

10

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Immunology in the medical area
  • Microbiology in the medical area

Keywords

  • molecular dynamics
  • linkers
  • lectins
  • carbohydrates
  • click chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 1439-4227