Engineered nanomaterials in in vivo and in vitro models
Summary, in English
We have used enhanced darkfield microscopy – hyperspectral imaging method to detect and identify ceria nanoparticles in three differently prepared samples, and compared different combinations of data analysis methods.
The remaining part of the thesis was devoted to the study of the III-V semiconductor NW effects on cells and tissue in vitro and in vivo. We have exposed two types of cell cultures (primary human lung (SAE) and human lung adenocarcinoma cell line (A549)) to gallium phosphide (GaP) NW. We observed that both type of cells are capable to engulf NW after 2 days, and that NW do not have any effect on the SAE and A549 cell morphology after 7 and 5 days, respectively. The NW concentrations used in this work are not toxic to A549 cells, since we did not see a change in cell mobility, viability or changes in ROS levels. After inhibiting specific endocytosis pathways in A549 cells, we showed that NW are actively taken up via phagocytosis and/or macropinocytosis.
Additionally, we have performed in vivo mice exposure to GaP NW via intratracheal instillation. We have observed that NW induce an inflammation and allergic reaction of similar levels as the ones observed after exposure to carcinogenic multiwall carbon nanotubes. Additionally, we show that NW are capable to cross tissue barriers and reach distant organs after 28 days, however without introducing changes to the organ tissues.
Lastly, we have performed a pilot study, where we investigated the effect of NW photodiodes on A549 cells. We observed that the presence of light inhibits cell proliferation. Furthermore, we show that the chemical make-up of the photodiodes is important, since some photodiodes dissolve in water based cell culturing media and release toxic compounds.
- Nano Technology
- III-V semiconductor nanowires
- Nanoparticle exposure
- Mouse exposure
- Cell exposure
- Fysicumarkivet A:2018:Abariute
- ISBN: 978-91-7753-824-0
- ISBN: 978-91-7753-825-7
19 October 2018
Rydbergsalen, Fysicum, Professorsgatan 1, Lund University, Faculty of Engineering LTH.