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The breast cancer-associated gene Di12 has oncogenic activity

På svenska

Author

  • AM Burger
  • R Grugel
  • Göran Landberg
  • R Kitching
  • A Seth

Summary, in English

The breast cancer-associated gene Di12 encodes a novel protein, which was found overexpressed in invasive ductal carcinomas of the breast. In experiments designed to assess the role of the Di12 gene in oncogenesis, the overexpression of 339 N-terminal amino acids of this gene in NIH3T3 cells resulted in cellular transformation and in vivo tumorigenesis. NIH3T3-Di12 tumor cell growth was partly reversible upon Di12 antisense treatment. In addition, transfortnation of the ER + human breast cancer cell line MCF- 7 resulted in hormone independent growth of these tumors in nude mice. Di12 expression in NIH3T3 and MCF-7 tumor cells was confirmed by RT-PCR and mabDi12 immunostaining. Immunohistochemistry using mabDi12 on an arrayed collection of 106 invasive breast tumors further underlined the expression of the gene in over 75% of advanced stage breast cancers. Our data indicate that Di12 expression is oncogenic in in vitro transformation and in vivo tumorigenic assays.

Department/s

Publishing year

2003

Language

English

Pages

2027-2033

Publication/Series

Anticancer research

Volume

23

Issue

3A

Document type

Journal article

Publisher

International Institute of Cancer Research

Topic

  • Cancer and Oncology

Keywords

  • nude mice
  • mabDi12
  • tumor formation
  • Di12
  • clonogenic assay
  • gene
  • expression

Status

Published

Research group

  • Pathology, Malmö

ISBN/ISSN/Other

  • ISSN: 1791-7530