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A fibroblast heparan sulphate proteoglycan with a 70 kDa core protein is linked to membrane phosphatidylinositol

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Author

Summary, in English

Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [35S]sulphate in the absence of serum. Cell cultures labelled with [3H]myo-inositol in the absence or presence of serum produce a radiolabelled heparan sulphate proteoglycan which was purified by gel-permeation chromatography and ion-exchange chromatography on MonoQ. Digestion with heparan sulphate lyase and analysis by gel-permeation chromatography and sodium dodecylsulphate-polyacrylamide gel-electrophoresis revealed that the 3H-label is associated with a core protein of apparent mass 70 kDa.

Publishing year

1990

Language

English

Pages

563-572

Publication/Series

Glycoconjugate Journal

Volume

7

Issue

6

Document type

Journal article

Publisher

Springer

Topic

  • Dermatology and Venereal Diseases
  • Pharmacology and Toxicology
  • Medicinal Chemistry

Keywords

  • heparan sulphate
  • phosphatidylinositol
  • fibroblasts
  • proteoglycan

Status

Published

Research group

  • Glycobiology

ISBN/ISSN/Other

  • ISSN: 1573-4986